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© 1994 American Society of Plant Physiologists
(from Durso & Cyr 1994, Plant Cell, 6: 893-905)
(A) Immunoblot analysis using antiserum raised against an EF1a homolog (ABP50) from Dictyostelium. The antiserum reacts monospecifically with pp50 in both the tubulin binding proteins (lane 1) and the tubulin/CaM binding proteins (lane 2).
(B) Indirect immunofluorescence microscopy using the anti-ABP50 serum characterized in (A). MT bundles were formed by mixing tubulin/CaM binding protein with taxol-stabilized MTs (as described in Figure 2A). These bundles were fixed, settled onto coated slides, and reacted with the anti-ABP50 serum during processing for indirect immunofluorescence microscopy. The fluorescence observed in (B) indicates that pp50 is localized to the MT bundles.
Bar= 10 µm.
