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© 1994 American Society of Plant Physiologists
(from Durso & Cyr 1994, Plant Cell, 6: 893-905)
(A) MT bundles resulting from the addition of tubulin/CaM binding proteins (enriched in pp50) to taxol-stabilized MTs. Bundles were observed by dark-field microscopy without fixation.
(B) The dissociation of the bundles shown in (A) into single MTs with the addition of Ca2+ plus CaM.
In controls, no effect on the bundles shown in (A) was observed when the suspension was adjusted to the equivalent concentrations of CaCl2 alone, or of CaM alone. In addition, none of these three treatments had an observable effect on taxol-stabilized brain MTs alone. In order to reduce light intensity to a level compatible with the video camera, the optical filtration required for obtaining the image shown in (A) was five times greater than that for (B). Bar= 10 µm.
Similar, but not identical, results have been found using carrot microtubules (see Fig. here).
