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© 1994 American Society of Plant Physiologists
(from Durso & Cyr 1994, Plant Cell, 6: 893-905)
Tubulin/CaM binding proteins were assayed for binding to carrot MTs using cosedimentation. Replicates of the cosedimentation pellet (lanes marked p) and supernatant (lanes marked s) were subjected to SDS-PAGE and transferred to nitrocellulose membrane. The membrane was temporarily stained for protein with Ponceau red and cut into three identical strips. Each of the three strips was independently immunoblotted using anti-ABP50 serum (EF-1a; first and second lanes), anti-alpha-tubulin (third and fourth lanes), or anti-beta-tubulin (fifth and sixth lanes).
