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Alignments of amino acid sequences of tryptic
peptide fragments of pp50 with corresponding sequences of EF1a
homologs.
pp50 was proteolytically digested and fragments were separated
by HPLC. The amino acid sequences for five selected fragments
are shown (top lines).
(A) Interspecies comparison of corresponding sequences
from tomato, Arabidopsis and mung bean, wheat, barley, Dictyostelium
discoideum, Mucor racemosus, Saccharomyces
cerevisiae, Euglena, Tetrahymena,
Artemia salina, sea urchin, Drosophila, Xenopus,
and human.
(B) Intraspecies comparison of other carrot EF1a sequences:
A/Z from unpublished sequence in the EMBL data bank; CEM1; PIK
is PIKA49.
Numbers correspond to the first amino acid residue in each fragment
shown; 000 denotes data derived from incomplete sequences. A
dot indicates identity with the top line (pp50 fragment sequence).
A question mark in pp50 sequences indicates that amino acid sequencing
did not permit unambiguous identification of the residue. The
two residues indicated by question marks are known to be posttranslationally
modified in some organisms.
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