| Date of Commission | Name and Rank | Remarks |
| Colonel | ||
| Feb 14, 1862 | John C. Rathbone | Honorably discharged to date Jan 6, 1863 |
| Feb 6, 1863 | Daniel Frost | KIA @ Snicker's Ferry, Va., Jul 19, 1864 (photo) |
| Oct 28, 1863 | Van H. Bukey | Mustered out. Ex. term service |
| Lieut. Colonels | ||
| Oct 17, 1861 | John C. Rathbone | Promoted to Colonel |
| May 24, 1862 | Daniel Frost | Promoted to Colonel |
| Jul 31, 1863 | Van H. Bukey | Promoted to Colonel |
| Feb 7, 1865 | William H. H. King |
| Majors | ||
| Nov 1, 1861 | George C. Trimble | Dismissed Jan 4, 1863 |
| Feb 26, 1863 | Van H. Bukey | Promoted to Lieut. Colonel |
| Nov 20, 1863 | James L. Simpson | Mustered out. Ex. term service |
| Feb 7, 1865 | Michael A. Ayres |
| First Lt & Adj't | ||
| Nov 13, 1861 | T. A. Roberts | Resigned, Oct 9, 1862 |
| Nov 5, 1862 | John H. MLaughlin | Discharged 5/18/1865 by wounds rec'd inaction at Cloyd's Mountain, Virginia (shoulder hit by shell) |
| First Lt & R. Q. M. | ||
| Jan 3, 1862 | David Bell | Mustered out. Ex. term service |
| Jan 12, 1865 | Hugh P. Dils, Jr., |
| Surgeon | ||
| Oct 27, 1862 | Andrew D. Voorheis | Resigned Oct 17, 1863 |
| Jan 12, 1865 | Erwin D.J. Bond |
| Assistant Surgeon | ||
| Aug 17, 1862 | Andrew D. Voorheis | Promoted to Surgeon |
| Mar 7, 1863 | James A. Williamson | Never mustered into service |
| Jan 5, 1864 | Erwin D.J. Bond | Promoted to Surgeon |
| Feb 8, 1864 | James Emory Kendall |
| Chaplain | ||
| Oct 10, 1863 | Aaron J. Lyon | Dismissed Dec 24, 1864 |