Fu Jen Catholic University Taipei, Taiwan
(Cumulative GPA 4.0)
Advanced Virology, Enzymology, Biotechnology, Nucleic Acid Chemistry, Molecular Biology, Cellular Biochemistry, Animal Cell Culture, Electron Microscopy, Techniques in Molecular Biology, Master Thesis
Bachelor of Science in Marine Biology (Aquaculture)
National Taiwan Ocean University,Keelung, Taiwan
June 1988 (Cumulative GPA 3.5)
Biology, Genetics, Microbiology, Physiology, Histology, Pathology, Psychology, Chemistry, Analytical Chemistry, Organic Chemistry, Biochemistry, Enzyme Chemistry, Calculus, Computer, Logic, Biometry, Physics, Experimental Design, Graduate Thesis
Workshop on Baculovirus Expression Vector Systems 1990
Viral and plasmid DNA transfection, baculovirus plaque assay, recombinant virus screening, cloning and amplification.
Workshop on Application of Recombinant DNA Technology in Fishery Sciences 1989
Nucleic acid methodology, cDNA and genomic library construction and screening, recombinant DNA technology application.
Workshop on Monoclonal Antibody and In Vitro Immunization 1989
In Vitro immunization, PEG cell fusion and cloning, ELISA, monoclonal antibody production, purification and cryogenic storage.
Employment:
2001-present
Senior Assay Development Associate
Cell Genesys Inc.
GVAX prostate/lung project:
Develop and validate cell-based and non cell-based assays to detect residues and potency for final products
Develop enzyme activity assays to ensure quality of components in process
Characterize and compare bioequvalence of Phase II/III and lot-to-lot products
Write AQP, AQR and draft SOP for assays
Organize and prepare samples for testing
Train, transfer assays to analyst of QC in GMP facility
Problem solving for QC assays
Coordinate studies with PD and various research groups
Conduct antigen discovery projects by using microarray and protein assays
Extract cellular protein from various organelles
Oncolytic virus therapies prostate project:
Develop assays to ensure purity plasmid DNA by cloning and sequencing
Design RT-PCR and restriction mapping assays to ensure insert DNA from mammalian cell lines
1996-2001
Medical Research Specialist
Dept. of Medicine
Tulane University Medical Center
Developed program for lab cell storage, chemical list
Managed grant accounts & purchasing
Cultured mammalian cell (proximal tubular cell, MDCK, etc.)
PCR Cloned antisense focal adhesion kinase gene into IPTG inducible vector
Transfected & subcloned stable inducible cell lines
Western blotting, Immunoprecipitation
Immunohistochemistry
Fluorescence Microscopy Image Analysis (Metaphore)
Flow Cytomery
Sequencing Computer analysis and search
Oligo-sequence analysis and primer design
1992-96
Graduate assistant
Dept. of Microbiology
Louisiana State University Medical Center
extracted Streptococcus DNA
Biotin gene labeling and protein expression
Isolated DNA by streptavidin magnetic beads
Dideoxynucleotide DNA sequencing
Scintillation Spectrometry
Accessed National Center for Biotechnology Information (NCBI) BLAST program and Ribosomal Database Project (RDP) gene databases
isoelectric focusing electrophoresis
1994-95
Laboratory technician
Dept. of Pathology- Bacteriology
Charity Hospital
Medical Center of Louisiana
Accessed & Analyzed specimen by customeized computer diagnosis software
incubated aerobic bacteria by Chocolate, Thayer-Martin, TSA blood agar, MacConkey agar; Campylobacter, Yersinia culture
Brain heart infusion broth bacteria storage
Bacti/Alert blood culture
Gram stain.
1990-92
Research assistant
Molecular Virology Lab.
Dept. of Biochemistry
College of Medicine
National Taiwan University
Cultured Cos7,HeLa,HepG2,Alexander cell
Subcloned stable cell line
Extracted cellular RNA , PCR cDNA synthesis
M13 cloning and sequencing
Rapid Amplification of cDNA Ends (RACE) by PCR
constructed Hepatitis Delta virus antigen cDNA shuttle vector
Electroporation
liposome transfection & LacZ histostain localization
Phosphor-Image Radioactivity analysis
1989-90
Graduate assistant
Molecular Virology Lab.
Dept. of Zoology
National Taiwan University
Constructed Baculovirus expression vector by PCR
Restriction Enzyme Mapping
CaCl2 co-transfection
Dot Southern Hybridization
Extracted cellular protein extraction
Purified viral RNA & cDNA synthesis
Spinner Cell Culture
1988-89
Graduate assistant
Cell Biology & Virology Lab.
Dept. of Biology
Fu Jen Catholic University
Cultured SF-21AE (insect) and TO-2 (fish ovary) cell lines
Inoculated Infectious Pancreas Necrosis Virus (IPNV)
CsCl gradient DNA purification
Electronic Microscope (TEM & SEM)
Dark Room techniques & development of photography of EM
1988
Research assistant
Enzymology & Nutrition Lab.
National Taiwan Ocean University,Keelung, Taiwan
analyzed nutritional requirements of artificial feed
Penaeus penicillatus hepatopancreastissue section
Bouin's H.E., Helly's AB-BFR staining
Histochemical enzyme localization
Protease and amylase activity assay.
1985
Research assistant
Taiwan Fisheries Research Institute
analyzed water quality
extracted gonadotropin-hypothalamus
induced maturation
anaesthetized fish
analyzed blood contents
bred nature feed
spawned seawater fish
fry transportation.
PROJECTS
1999-2000 System Analysis & Design
System definition & procurement of technology
Application Architecture & Process Design
Transform analysis
Process specification
SKILLS
COMPUTER
Operating Systems
Window 3.11/95/NT
UNIX (IBM RS6000, FreeBSD, Linux, IBM VAX)
Macintosh
MS-DOS
Languages & Softwares
FORTRAN BASIC C/C++ Java JavaScript HTML
ERWIN
Word Perfect,Personal Oracle 8i,Access Microsoft Office (Word, Excel, PowerPoint),
Aldus PageMaker, Aldus Publisher, PhotoShop, Lotus Improv, Corel Draw, Statistics, SAS,
Vector NT, Primer, PCGENE, GeneBank Sequencing Computer Analysis,
Laboratory techniques:
Quantitative Flow Cytometry, Luminex 100, cell-based bioactivity assays, cytotoxicity assays, Thin-prep 2000, ELISA
TaqMan PCR, Restriction Enzyme Mapping, Bacterial phage DNA Purification, mammalian cell RNA purification,
Southern/Northern Hybridization
OTHERS
Fluent in English, Mandarin, Taiwanese and basic Japanese
Publication:
Growth factors stimulate kidney proximal tubule cell migration independent of augmented tyrosine phosphorylation of focal adhesion kinase (FAK). Biochemical and Biophysical Research Communications 328: 560-566 (2005).
The effect of growth factors on the phosphorylation of focal adhesion kinase (FAK) in renal tubule cells. Southern Society for Clinical Investigation, New Orleans, New Orleans, LA, February, 2003
Effect of oxidant stress on growth factor stimulation of proliferation in cultured human proximal tubule cellsKidney International Volume 56 Issue 4; October 1999
Abstract
Effect of oxidant stress on growth factor stimulation of proliferation in cultured human proximal tuble cells.
American Society of Nephrology 31st Annual Meeting, Oct. 1998
Abstract
Effect of sodium entry on Na-K-ATPase protein and mRNA in M-1 cells.
American Society of Nephrology 31st Annual Meeting, Oct. 1998
Abstract
Bacteriology of urinary bladder tissue and urine in interstitial cystitis. Gerald J. Domingue, Gamal M. Ghoniem, Liset Human, Kenneth Johnston, Kuoting Wu.
American Urological Associate Annual Meeting 1996
Abstract
Master thesis: (Louisiana State U. Medical Center)
Approaching A Method to Detect the Etiologic Agents of Interstitial Cystitis
Abstract
Introduction
Material & Methods
Results
Discusstion
Tables
Nuclear Localization Signals, but Not Putative Leucine Zipper Motifs, Are Essential for Nuclear Transport of Hepatitis Delta Antigen
Journal of Virology Vol.66(10): p6019-6027 , Oct. 1992
Abstract
Master thesis: (Fu Jen Catholic U.)Amplification of Autographa californica Polyhedrin Gene LeaderSequence by Polymerase Chain Reaction for ConstructingBaculovirus Vectors
Abstract
HONORS
Tulane University, Dean's List of 2000
Tulane University, Dean's List of 1999
Scholarship of Li-Ching Foundation, Taiwan 1990
Scholarship of Ministry of Education, Taiwan 1984-85
Activities:
Coordinator of National Taiwan Ocean U. Orchestra, Swimming Team, Photographer of Tropical Fish Research Group
LSU Medicine History Club, New Orleans Area Virology Association
Membership:
American Society For Microbiology, Taiwan Society For Microbiology , ROC., Taiwan Society For Entomology, ROC., Taiwan Diving Association
Interests:
Web desinger, play trumpet , swimming, diving, interacting with people, stone sculpture, philatelist, audio and classic music lover, photography and calligraphy learner
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