| Years?#060;/TD>
| Positions?#060;/TD>
| Duties and achievements?#060;/TD>
|
?965-1967?
?
?#060;/TD>
| Lieutenant in the regular army in the 16th Group of Personnel
carrier.(16th Groupe de Chasseurs Portés)?#060;/TD>
| .?#060;/TD>
|
| 1970?#060;/TD>
| Medical Technologist in hematology and Tissue Typing?
(Paris France)?#060;/TD>
|
Duties:
Long term freezing of viable lymphocytes in liquid nitrogen. HLA Class
I typing of platelets.?
HLA Class I typing of lymphocytes?#060;/TD>
|
1971-1974?
?#060;/TD>
| Technical officer in the Tissue Typing serology laboratory,
NSW Red Cross Blood Transfusion Service Sydney.?
?#060;/TD>
|
Duties:
_To set up cryogenic techniques which were acquired overseas.?
_Preparation of serum for screening and crossmatching.?
_HLA Class I tissue typing using the complement dependent microlymphocytotoxicity
test, interpretation and recording of results.?
_General laboratory duties.?
Achievements:?#060;/H5>
_Study of the HLA system in expatriate Lebanese domiciliated in Sydney,
and in the Australian Aboriginal population. This work was published in
two articles in histocompatibility Testing, 1972 (see list of?
publications).?#060;/TD>
|
| 1975-1978?#060;/TD>
| Technical officer in the Tissue Typing laboratory, NSW Red
Cross Blood Transfusion Service Sydney.?#060;/TD>
|
Duties:?#060;/H5>
_Issuing of tissue typing reports.?
_Micro-agglutination test of leukocytes from donor/patient pairs?
_On call for typing of potential kidney donors.?
_Preparation of cross match trays.?
_Preparation of HLA class I grouping trays.?
_Preparation of screening trays.?#060;/TD>
|
| 1978-1983?#060;/TD>
| Technical officer Grade II in the Tissue Typing laboratory,
NSW Red Cross Blood Transfusion Service Sydney.?#060;/TD>
|
Duties:?#060;/H5>
_Class II serological typing of B Lymphocytes using the complement dependent
microlymphocytotoxicity test.?
_On call for typing of organ donors.?
_Screening for HLA class II antibodies.?
_Development of the pooled platelet absorption method used for the
preparation of anti-sera in HLA-DR typing.?
_Programming of a laboratory data base using Fortran for family data
management that was in use until 1993.?
_Programing of a statistical package using Fortran for analysis of
populations by the maximum likelihood method and Chi square analysis, the
program is now also written in a PC version.?
Achievements:?
_Statistical analysis of HLA Class I and Class II alleles in a Sydney
Jewish population (published).?
_Idiopathic haemochromatosis study in the Australian population (Co-author:
published).?#060;/TD>
|
| 1983-1997?#060;/TD>
| |
Duties:
_Class II serological typing of B Lymphocytes using the complement dependent
microlymphocytotoxicity test.?
_On call for typing of organ donors.?
_Screening for HLA class II antibodies.?
_Development of the pooled platelet absorption method used for the
preparation of anti-sera in HLA-DR typing.?
_Programming of a laboratory data base using Fortran for family data
management that was in use until 1993.?
_Programing of a statistical package using Fortran for analysis of
populations by the maximum likelihood method and Chi square analysis, the
program is now also written in a PC version.?
Achievements:?#060;/H5>
_Statistical analysis of HLA Class I and Class II alleles in a Sydney Jewish
population (published).?
_Idiopathic haemochromatosis study in the Australian population (see
publications).?#060;/TD>
|
| 1983-1994?#060;/TD>
| Hospital Scientist level 6-8 in the DNA Laboratory, Tissue
Typing,?
NSW Red Cross Blood Transfusion Service, Sydney.?
?#060;/TD>
|
Duties:
_Second in charge of the laboratory.?
_Checking and issuing of test results.?
_Application of the restriction fragment length?
polymorphism (RFLP) technique to HLA-DR typing for routine laboratory
use. The same technique was also applied to HLA-DPB, -DQ, -DX, and -DOB
typing.?
_Introduction of polymerase chain reaction (PCR) based techniques such
as sequence specific oligonucleotide (SSO) typing, and amplified fragment
length polymorphism?
(AFLP) typing for the determination of HLA-DRB1, -DRB3, -DRB4, -DRB5,
-DP, -DQB1, and DQA1 typing.?
_Monitoring of the DR and DQ subtyping methods to maintain the highest
degree of subtyping possible. This also involves the development of methods
to apply sequence?
specific priming (SSP) as a problem solving technique in HLA class
II subtyping.?
_Programming of an algorithm for the determination of HLA alleles from
restriction enzyme results.?
_Programming of a Laboratory data base to store typing results and
issue reports.?
Achievements:?#060;/H5>
_The success obtained in DNA class II typing has resulted in the routine
use of these techniques in matching for organ transplantation.?
_Participation in the collaborative transplant study (CTS) organised
by Professor G. Opelz (Germany) which has lead to several publications.?
_A Population study involving DNA-DR and -DQ typing of?Papua
New Guineans and Australian Aborigines (published).?
_Development of HLA-DRB5 typing (published) and HLA-DRB3 typing using
the AFLP technique.?
_Discovery of new sequences in the HLA-DR and -DQ region (submitted).?
_Further studies are being carried out examining the HLA class II genes
in various Asian and South East Asian populations which will be of benefit to patients
from these ethnic groups who require transplantation.?#060;/TD>
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