Control of Aspergillus flavus Link. and Aflatoxin on Corn Seeds under Storage Condition by Medicinal Plant Extracts

Porntip Chompooming

 

Kasetsart University, Chatuchak, Bangkok 10900

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Thirty-three crude extracts at 5000 ppm by solvent extraction using dichloromethane, n-hexane and methanol from 11 medicinal plant spicies were screened by poisoned food technique against Aspergillus flavus.  Eight crude extracts had effective inhibition of the pathogen and were used for retest at different concentrations.  The results indicated that clove tree extracts using dichloromethane and n-hexane were most effective in A. flavus growth inhibition with ED₅₀ of 4.40 and 13.32 ppm, respectively.  However, in comparing to benomyl fungicide, there was no significant difference.

In case of controlling storage mold and reducing the contamination of aflatoxin on corn seeds using the extracts with ED₅₀<500 ppm, only the clove tree extracted by n-hexane and the cinnamon tree extracted by dichloromethane at the concentration of 500 ppm were satisfactorily effective than the control. 

 

 

 

Porntip