Growth
Promotion of in vitro Maize (Zea mays L.) plantlets under
Photoautotrophic Condition
Parinya Khajudparn1, Anongnut Bhonwong1, Chaba Jampatong2, and Piyada Thipyapong1
1. School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology.
2. National Corn and Sorghum Research Center, Kasetsart University.
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Growth of in
vitro maize plantlets cultured on MS medium photoautotrophically with
vermiculite as a supporting material was compared with those cultured
photomixotropically with vermiculite and agar (control) as supporting
materials. The leaf and root
lengths, number of roots, leaf and root fresh weight, and leaf and root dry
weight were recorded on days 3, 5, 7, and 9 of culture. Plantlets grown photoautotropically had the highest leaf and
root lengths, and leaf and root fresh weight at all stages of growth (days 3,
5, 7, and 9; p<0.05). On day 9
they had 1.5-, 1.7-, 1.7-, 4.1-, and 1.6- fold higher leaf and root lengths, leaf
and root fresh weight and number of roots than control, respectively. Under photomixotrophic condition, using
agar as a supporting material led to higher number of roots since day 5
(p<0.05) and on day 9 leaf fresh weight was 1.3-fold higher than using
vermiculite (p<0.05). Therefore, using vermiculite as a supporting material
promotes growth of in vitro maize plantlets under
photoautotrophic condition, but may inhibit growth under photomixotrophic
condition. The increase in length
and number of roots under phototrophic condition may enhance survivability
during ex vitro acclimatization.
ChabaDNA