Research Associate
University of Iowa, Department of Chemistry, Iowa City, IA 52242
Anatoly V. Chernyshev, PhD (chemistry)
Research Associate
University of Iowa, Department of Chemistry, Iowa City, IA 52242
Institutions Attended
1989–1994
MSc
Organic Chemistry, Saratov State University, Russia
1994–1999
PhD
Physical Chemistry, Saratov State University; Institute of
Physiology and Biochemistry of Plants and Microorganisms, Russian Academy of
Sciences, Russia
Postgraduate
Education
24th Int. School of Crystallography “Experimental and
Computational Approaches to Structure Based Drug Design”, Erice, Italy, 9-19
May 1996.
27th Int. School of Crystallography “Implications of Molecular and
Materials Structure for New Technologies”, Erice, Italy, 28 May-7 June 1998.
Professional and Academic Positions Held
September 2003 — Present
Research associate at Chemistry Dept., University of Iowa, Iowa City, USA.
Study of heavy kinetic isotope effects in nitrogenase enzyme reactions; study of
flavin-dependent thymidylate synthase.
January 2007 — May 2007
Visiting Assistant Professor at
Chemistry Dept., University of Iowa, Iowa City, USA. Teaching lecture course on
physical chemistry (thermodynamics).
March 2001 — August 2003
Research associate at Physiology dept., Loyola University, Chicago, USA.
Study of peptide proton channels (gramicidin A); molecular mechanic modeling of
proton current.
August 94 — March 2001
Research Associate, Laboratory of Biochemistry, Institute of Biochemistry
and Physiology of Plants and Microorganisms Russian Academy of Sciences,
Saratov, Russia. Study of glutamine synthetase enzyme; study of biogeochemical
formation of mineral struvite.
June 2000 — March 2001
Senior Chemical Ecology Engineer at the Scientific Center "EMOS",
Saratov, Russia. Chemical ecological assessment of gas and oil deposits
territories in East Siberia.
September 1995 — December 2000
Lecture course “Structure and functions of biomacromolecules” at the
Department of Chemistry, Saratov State University, Russia.
Summary
of formal qualifications
Protein purification; enzyme kinetics; UV-vis, IR-, fluorescence, atomic
absorption, atomic emission spectroscopy; isotope ratio mass spectrometry
(IRMS); single channel recording (peptide ion channels); manipulation of
air-sensitive substances and gases; vacuum lines; quantum chemical and molecular
mechanics calculations (programs MOPAC, Gamess, Gaussian, AMSOL); protein
docking simulations (programs DOCK, Autodock); Cambridge Structural Database.
Languages: Russian (native), English.
Honors and Awards
1997, 1998
The Soros graduate student (http://www.soros.org).
2005
InnoCentive Challenge award “Preservative degradation”
February 2006
InnoCentive Challenge award “Improving UV resistance of coating
containing metal ions”
April 2006
InnoCentive Challenge award “Biological targets”
March 2007 InnoCentive
Challenge award “Cellulose Pyrolysis”
May 2007
InnoCentive Challenge award “ALS Biomarker”.
Graduate Students Supervised
1996–1998
Moksin S. A., M.Sc., “Interaction of metal ions with glutamine
synthetase and bovine serum
albumin”, Saratov State University (advisor in chemistry).
1996–1998
Kutuzova E. V., M.Sc., “Electrochemical study of phosphatase activity
of lanthanides and glutamine synthetase
reactions”, Saratov State University (advisor in chemistry).
1996–1998
Kosterina N. V. “QSAR analysis in the series of chalcogenopirilium
salts and 1,5-diketones”, Saratov State University (advisor in chemistry).
2005
Wilmarie Santana (SROP fellowship student from University of Puerto
Rico-Bayamon).
2007–present
Zhen Wang (graduate student).
Areas of Research Interest and Current Projects
Mechanistic enzymology; protein biophysics; bioinorganic chemistry; isotope effects; drug design.
Current Projects
1. Design of an inhibitor for flavin-dependent thymidylate synthase (postdoc).
2. Biomarker for amyotrophic lateral
sclerosis (PI).
3. Drug design for vitiligo therapy
(freelance).
Membership in Professional Organizations
1997–present
The Mendeleyev Russian Chemical Society.
2001–present
The Biophysical Society (USA)
2003–present
The American Chemical Society.
2003–present
The International Union of Pure and Applied Chemistry (IUPAC; Affiliate
member)
Publications
Papers:
1. Antonyuk L. P., Kamnev A. A., Chernyshev A. V., Ignatov V. V. The
formation of struvite crystals during cultivation of the soill bacterium Azospirillum
brasilense//Doklady Akademii Nauk (Proceedings of the Russian Academy of
Sciences).-1996.- V. 350.-N3.- P. 421-423.
2. Kamnev A.A., Antonyuk L.P., Chernyshev A.V., Ignatov V.V., Colina de Vargas
M. Spectroscopic characterization of mineral crystals produced by the bacterium Azospirillum
brasilense//Fresenius J. Anal. Chem.- 1996 - V. 355 - P. 739-741.
3. Kamnev A.A., Ristic M., Antonyuk L.P., Chernyshev A.V., Ignatov V.V. Fourier
transform infrared spectroscopic study of intact cells of the nitrogen-fixing
bacterium Azospirillum brasilense //J. Mol. Struct.- 1997 - V. 408/409 -
P. 201-205.
4. Kamnev A.A., Renou-Gonnord M.-F., Antonyuk L.P., Colina M., Chernyshev A.V.,
Frolov I., Ignatov V.V. Spectroscopic characterization of the uptake of
essential and xenobiotic metal cations in cells of the soil bacterium Azospirillum
brasilense //Biochem. Mol. Biol. Int.- 1997.- V. 41.- N 1.- P. 123-130.
5. Kamnev A.A., Ristic M., Colina M., Antonyuk L.P., Chernyshev A.V., Ignatov
V.V. Comparative spectroscopic study of intact cells and cell membranes of the
bacterium Azospirillum brasilense //In: Spectroscopy of Biological
Molecules: Modern Trends (P. Carmona, R. Navarro, A. Hernanz, eds.).-Dordrecht:
Kluwer Acad. Publ.- 1997.- P. 313-314.
6. Kamnev A.A., Antonyuk L.P., Colina M., Chernyshev A.V., Ignatov V.V. Physicochemical
investigation of a microbially produced modification of magnesium-ammonium
orthophosphate //Monatsh. Chem. (Chemical Monthly).- 1999 - V. 130 -
P. 1431-1442.
7. Chernyshev A. V., Cukierman S. A thermodynamic view of activation energies of
proton transfer in various gramicidin A channels.// Biophys. J.- 2002.- V.
82.- P. 182-192.
8. Chernyshev A. V., Pomes R., Cukierman S. Kinetic isotope effects of proton
transfer in aqueous and methanol containing solutions, and in gramicidin A
channels.//Biophys. Chem.- 2003. – V.
103. – P. 179–190.
9. Chernyshev A. V., Armstrong K. M., Cukierman S. Proton Transfer in
Gramicidin Channels is Modulated by the Thickness of Monoglyceride Bilayers.//
Biophys. J. - 2003- V. 84 - P. 238 -250.
10. Chernyshev A. V., Cukierman S. Proton Transfer in Gramicidin Water Wires in
Phospholipid Bilayers. Attenuation by Phosphoethanolamine.// Biophys. J.-
2006.- V. 91 – P. 580-587.
11. Chernyshev A.V., Fleischmann T., Kohen A. Thymidyl
Biosynthesis Enzymes as Antibiotic Targets (Mini-review)// Applied
Microbiology and Biotechnology.- 2007.- V. 74.- P. 282-289 (DOI
10.1007/s00253-006-0763-1).
12. Chernyshev A.V., Fleischmann T., Koehn E., Lesley S.A., Kohen A. The
relationships between oxidase and synthase activities of flavin dependent
thymidylate synthase (FDTS) // Chem. Comm. 2007.- P. 2861-2863 (DOI 10.1039/b700977a).
Also
featured in Chemical Biology. 2007.- N 8.
13. Chernyshev A.V., Sra A., Kohen A. Discriminating the nitrogenase mechanisms:
nitrogen KIE approach // (in preparation).
Lecture
courses:
1. Chernyshev A.V. “Structure and functions of biomacromolecules”. In:
Lecture courses and practices in bioorganic chemistry (Schegolev S. Yu.,
Astakhova L.N. Eds.) 2-nd ed., Saratov State University Press, 2000 (In
Russian).
Abstracts
of presentations:
1. Chernyshev A. V. Effects of levelling of Fermi energy in multimeric protein
associations //Abstr. 3rd IUBMB Conf. «Molecular recognition».-
23-27 Apr. 1995.- Singapore.-P. 115.
2. Chernyshev A. V., Antonyuk L. P., Bespalova L. A., Ignatov V. V. Metal
specificity of Azospirillum brasilense glutamine synthetase //Ibid.-P.
156.
3. Chernyshev A. V. Alternating Structures in Biology//Abstr. 3rd
Int. Symp. on Bioorganic Chemistry, Dagomys, Russia, 17-23 Sept. 1995, P. 29.
4. Kamnev A.A., Antonyuk L.P., Chernyshev A.V., Ignatov V.V., Colina de Vargas
M. Spectroscopic characterization of mineral crystals produced by the bacterium Azospirillum
brasilense //Abstr. XXIX Colloquium Spectroscopicum Internationale.- 27
Aug.- 1 Sept. 1995.- Leipzig, Germany.- P. 492.
5. Bespalova L. A., Chernyshev A.V., Antonyuk L.P., Ignatov V.V. Glutamine
synthetase of Azospirillum brasilense: influence of the activating metal
on the enzyme properties //Abstr. 10th Int. Congr. on Nitrogen
Fixation.- 28 May-3 June, 1995.- St.-Petersburg, Russia - P. 113.
6. Chernyshev A. V. Thermodynamical Attractors of Live Systems as a Possible Key
to Elucidation of New Drugs//Abstr. 24th Int. School of
Crystallography `Experimental and Computational Approaches to Structure Based
Drug Design', Erice, Italy, 9-19 May 1996, P10.
7. Chernyshev A. V., Kosterina N. V., Antonyuk L. P., Ignatov V. V. Influence of
triphenylsubstituted pirilium, thiopirilium and selenopirilium salts on the
catalytic activityof a bacterial glutamine synthetase //Abstr. 17th
Int. Conf. on the Organic Chemistry of Sulphur.- 7-12 July, 1996.- Tsukuba,
Japan.- P. 29.
8. Chernyshev A. V., Moksin S. A., Kutuzova E. V., Antonyuk L. P., Ignatov V. V.
Interaction of glutamine synthetase from the soil bacterium Azospirillum
brasilense with ions of lanthanides and uranyl (VI) ion// Abstr. Int.
Symp. on Subsurface Microbiology.- 15-21 Sept. 1996 - Davos, Switzerland.- P.
80.
9. Chernyshev A. V., Kutuzova E. V., Moksin S. A., Antonyuk L. P., Ignatov V. V.
The behaviour of glutamine synthetase from the soil bacterium Azospirillum
brasilense with ions of lanthanides //Abstr. 7th Int.
Symp. «Nitrogen Fixation with Non-Legumes».- 16-21 Oct. 1996 - Faisalabad,
Pakistan.- P. 16.
10. Kamnev A.A., Chernyshev A.V., Antonyuk L.P., Colina M., Ignatov V.V.
Involvement of metal cations by the soil bacterium Azospirillum brasilense in
the processes of its metabolism //Abstr. 26th Int. Symp. on
Environmental Analytical Chemistry.- 9-12 Apr. 1996 - Vienna, Austria.- P. 35.
11. Kamnev A.A., Ristic M., Antonyuk L.P., Chernyshev A.V., Ignatov V.V. Fourier
transform infrared spectroscopic studies of intact cells of the nitrogen-fixing
bacterium Azospirillum brasilense and some model compounds related to its
metabolism//Abstr. XXIII Eur. Congr. on Molecular Spectroscopy.- 25-30
Aug. 1996.- Balatonfüred, Hungary.- P. 122.
12. Kamnev A.A., Antonyuk L.P., Colina M., Chernyshev A.V., Ignatov V.V.
Assimilation of essential and xenobiotic metal cations by the associative
nitrogen-fixing bacterium Azospirillum brasilense//Abstr. 8th
Int. Congr. of Bacteriology and Applied Microbiology Division, IUMS.- 18-23 Aug.
1996.- Jerusalem, Israel.- P. 104.
13. Moksin S. A., Chernyshev A. V. Interrelationships between Primary and
Quaternary Structures of Heteropolymers Originated from Different Alphabets//The
FASEB J. 1997. V. 11. N9. P. A838.
14. Chernyshev A. V. Combinatorial Analysis of Complex Systems//The FASEB J.
1997. V. 11. N9. P. A838.
15. Fomina O. R., Chernyshev A. V., Kutuzova E. V., Konoplyantseva N. A.
Electrochemical investigation of the kinetics of a bacterial glutamine
synthetase//The FASEB J.- 1997- V. 11.- N 9.- P. A1140.
16. Kutuzova E. V., Fomina O. R., Konoplyantseva N. A., Chernyshev A. V. An
electrochemical investigation of the kinetics of glutamine
synthetase purified from Azospirillium brasilense.// Mol. Biol. Cell -
1997 - V. 8 - P 1648.
17. Chernyshev A. V. A theoretical study on the long-range protein-protein and
cell-cell interaction.//Mol. Biol. Cell - 1996 - V. 7 - P. 1648.
18. Chernyshev A. V. A Combinatorial Approach for Investigation of Assembled
Systems//Abstr. 2nd Ann. European Conf. on Combinatorial
Technologies, 4-6 Feb. 1998, Montreux, Switzerland, P. 5.
19. Chernyshev A. V., Kutuzova E. V., Kutuzov S. V., Fomina O. R.,Konoplyantseva
N. A. An electrochemical investigation of glutamine synthetase reactions//Abstr.
7th Eur. Conf. on Electroanalysis.- 24-28 May 1998 - Coimbra,
Portugal.- P. 43.
20. Chernyshev A. V. A Matter Study at the Microscopic Level Using
Combinatorics// Abstr. 27th Int. School of Crystallography
`Implications of Molecular and Materials Structure for New Technologies', Erice,
Italy, 28 May-7 June 1998, P10.
21. Chernyshev A. V., Fomina O. R., Kutuzova E. V., Moksin S. A., Konoplyantseva
N. A. Role of the ions of f-elements in biological systems.
Electrochemical and colorimetric study on interaction of a bacterial glutamine
synthetase and bovine serum albumine with lanthanides and uranyl (VI) ions//Abstr.
Eur. Conf. «Bacterial-Metal/Radionuclide Interaction: Basic Research and
Bioremediation».- 2-4 Dec. 1998 - Rossendorf, Germany.- P. 67-68.
22. Chernyshev A. V., Fomina O. R., Kutuzova E. V., Moksin S. A. Catalysis
performed by a bacterial glutamine synthetase in the presence of ions of s-,
p- and d-elements//Ibid.- P. 60-62.
23. Chernyshev A., Cukierman S. Temperature dependence of H+, D+
and K+ current through the native and covalently linked gramicidin A
channels. 46th Biophysical Society Annual Meeting, San Francisco, USA, February
23-27, 2002.
24. Chernyshev A., Armstrong K., Cukierman S. Bilayer Thickness Affects H+
Current through the Native and Covalently Linked Gramicidin A Channels. 47th
Biophysical Society Annual Meeting, San Antonio, USA, March 1-5, 2003 //
Biophys. J. - 2003 - V. 84 - No 2 - P. 94a.
25. Sra A., Chernyshev A., Kohen A. 15N Kinetic Isotope Effects in
Nitrogenase Reaction. Gordon Research Conference “Nitrogen
Fixation”, New London, NH, USA, June
20-25, 2004.
26. Fleischmann T., Chernyshev A.V., Kohen A. Mechanistic aspects of
flavin-dependent thymidylate synthetase. XXVI
Midwest Enzyme Chemistry Conference, Chicago, USA, September 30, 2006.
27. Chernyshev A.V., Koehn E., Fleischmann T., Wang Z., Lesley S.A., Kohen A. Thermotoga
Flavin Dependent Thymidylate
Synthase (FDTS): Implications for Oxygen Metabolism. Thermotoga 2007 Workshop,
La Jolla, USA, July 9-10, 2007.
28. Wang Z., Chernyshev A.V., Fleischmann T., Lesley S. A., Kohen A. The
Competition between O2 and 5,10-Methylene-5,6,7,8-Tetrahydrofolate
(CH2THF) in the Oxidative Half Reaction of Flavin-Dependent
Thymidylate Synthase (FDTS). XXVII Midwest Enzyme
Chemistry Conference, Chicago, USA, September 29, 2007.