217 Department of Biochemistry
Ullmann Building, c/o prof. D.Wallach group
The Weizmann Institute of Science
Rehovot 76100 ISRAEL
Tel: +972-89342138 or +972-89343753, Fax: +972-89343165
Email: yury.goltsev@weizmann.ac.il
http://www.geocities.com/bmyury/science
 

EDUCATION:

1989-1993:	Bachelor of Science Moscow Institute for Physics and Technology Majors in Physical Chemistry and Biophysics
1993-1995:	Master of Science  Moscow Institute for Physics and Technology  Subject area: Molecular Biophysics
1995-2002:	Ph.D. in Molecular Biology The Weizmann Institute of Science  Supervisor: Prof. David Wallach  Thesis topic: Novel approaches to identification of signaling molecules activated by receptors of the TNF/NGF family, and their application to the study of cFLIP/CASH

 
 

DATE OF BIRTH: June 10th, 1972
 

MARITAL STATUS: married, two children
 

CITIZENSHIP: Israel
 
 

David Wallach, Ph.D.
Professor,
Dept. Biological Chemistry
The Weizmann Institute of Science
Rehovot 76100 ISRAEL
Tel: 972-8-9343941 (or -9343753))
Fax: 972-8-9343165 (or -9344112 or -9468256)
E-mail : David.Wallach@weizmann.ac.il
http://www.weizmann.ac.il/Biological_Chemistry/scientist/Wallach/david_wallach.html

Stefan Leu, Ph.D.
Senior Scientist,
Dept. Biological Chemistry
The Weizmann Institute of Science
Rehovot 76100 ISRAEL
Tel: 972-8-9342138 (or -9343753))
Fax: 972-8-9343165 (or -9344112 or -9468256)
E-mail : bmleu@wicc.weizmann.ac.il

Shulamit Michaeli, Ph.D
Associate Professor
Faculty of Life Sciences
Bar-Ilan University
Ramat-Gan 52900, Israel
Tel: 972-3-5318068
Fax: 972-3-5351824
E-mail: michaes@mail.biu.ac.il
http://www.biu.ac.il/LS/staff/michaeli.html

Last six years:Molecular analysis of cytokine-induced signal transduction pathways, in particular those triggered by the TNF/FAS-family members. Molecular regulation of apoptosis.

Recombinant DNA: conventional molecular cloning techniques, RT-PCR, site-directed mutagenesis, construction of cDNA libraries, DNA & RNA expression analysis (electrophoresis, hybridization, protection, etc), library screening, subtraction by Representation Difference Analysis (RDA)
 

Mammalian Cell Culture: routine, expression of recombinant protein in culture cells (both transient & stable), analysis: Western, Far Western, immunocytochemistry, GFP visualization system, essential flow cytometry and sorting, ELISA.
 

Signaling-related Techniques: EMSA & reporter assays for transcription, in vitro kinase assay (in gel & in solution), cell viability assays (biochemical and visual), in vivo labeling, in vivo signal transduction pathway reporting systems (e.g. PathFinder from Stratagene).
 

Protein-protein Interactions Assays: yeast two- and three-hybrid systems; mammalian interaction traps, in vitro pull-down, in vivo co-immunoprecipitation (both endogenous and transfected proteins).
 

Proteomics: Tandem Affinity Purification (TAP) of protein complexes and identification of the components by microsequencing.
 

Bacterial Expression: Introduction and affinity purification of recombinant proteins, essential HPLC (gel filtration, ion exchange)
 
 

Boldin, M. P., Goncharov, T. M., Goltsev, Y. V., and Wallach, D. (1996). Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death, Cell 85, 803-15.
 

Goltsev, Y. V., Kovalenko, A. V., Arnold, E., Varfolomeev, E. E., Brodianskii, V. M., and Wallach, D. (1997). CASH, a novel caspase homologue with death effector domains, J Biol Chem 272, 19641-4.
 

Wallach, D., Boldin, M., Goncharov, T., Goltsev, Y., Mett, I., Malinin, N., Adar, R., Kovalenko, A., and Varfolomeev, E. (1996). Exploring cell death mechanisms by analyzing signaling cascades of the TNF/NGF receptor family, Behring Inst Mitt, 144-55.
 

Wallach, D., Varfolomeev, E. E., Malinin, N. L., Goltsev, Y. V., Kovalenko, A. V., and Boldin, M. P. (1999). Tumor necrosis factor a receptor and Fas signaling mechanisms, Annu Rev Immunol 17, 331-67.