Lemurs reach sexual maturity between eighteen months and two years of age. Most females within the troop go into estrus during a 24 to 48 hour period, between May and July. During this time the male of the troop must impregnate all of the members of the troop or else it will be another entire year before the chance arises again. The gestation period last between 90-102 days, and each female lemur has a litter of up to six, but most commonly three babies. Lemurs are the only primates to have litters. They also do not carry their offspring on their stomachs or backs, but rather leave their litters in nests of leaves.
Black-and-white ruffed lemurs have a matriarchal social order, which means the females are dominant over the males. This is why the zoo's two females are in separate cages, as they will fight for dominance if placed together. Each troop is generally constructed of one adult female, one adult male, and their children, however some troops have been known to contain more that one adult female. Males generally leave the troop at sexually maturity, where females either fight for dominance, stay as a non-dominate member, or leave to start their own troop. Social bonding is strengthened through mutual grooming. Since Black-and-White Lemurs generally don't have opposable thumbs like other primates, they use their lower front teeth, which are horizontal and comb-like. They also have large toilet claws on each of their hind feet. The lemurs in the zoo probably don't have the same kind of social bonds as wild lemurs, since they are live alone and only experience mutual grooming from their keepers.