© 1994 Springer-Verlag. Reproduced with permission. |
An EF-1a homolog from carrot bundles plant microtubules (MTs), and calcium/calmodulin (Ca2+/CaM) abolishes the bundling.
These images are darkfield micrographs - direct and rapid observations of MTs without fixation, fluorescent labeling, mounting, etc. Using only purified components reconstituted in vitro:
At present (1996), this and related data are the most detailed functional characterization of a putative microtubule-associated protein in plants. Our subsequent in situ studies indicate that EF-1a is a bona fide, not putative, microtubule-associated protein.
Details:
|
©1996 Neil A Durso, III |
|