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© 1996 Springer-Verlag. Reproduced with permission.
(from Durso et al. 1996, Protoplasma 190: 141-150)
Dual immunolocalization using conventional epifluorescence microscopy on a phragmoplast of a taxol-treated carrot protoplast lysed prior to fixation is shown. Fluorescein fluorescence reporting affinity-purified anti-pp50 antibodies (a) is highly similar to Texas Red fluorescence reporting anti-carrot tubulin mAb1F8 (b), indicating that pp50's co-localization with microtubules is not exclusive to the interphase cortical array. Bar = 4 um.
