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© 1996 Springer-Verlag. Reproduced with permission.
(from Durso et al. 1996, Protoplasma 190: 141-150)
The suspension was settled onto poly-L-lysine-coated slides and processed for immunofluorescence microscopy using (a) anti-soy tubulin (1:100), or (b) affinity purified anti-pp50 antibodies (1:1). Similar to b, unpurified anti-pp50 serum (1:100) was negative (not shown). Note that anti-pp50 serum or affinity purified anti-pp50 antibodies do not recognize microtubules of pure carrot tubulin in this immunolocalization protocol.
