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© 1994 American Society of Plant Physiologists
(from Durso & Cyr 1994, Plant Cell, 6: 893-905)
Total soluble proteins from carrot cells (lane a) were chromatographed using a matrix of covalently immobilized tubulin. Proteins that required 0.3 M NaCl for elution from the matrix were designated 'tubulin binding proteins' (lane b). These proteins were assayed by cosedimentation with taxol-stabilized MTs free in solution to determine which tubulin binding proteins are also MT binding proteins. Proteins that did not bind MTs tightly remained in the cosedimentation supernatant (lane c) when MTs were pelleted by centrifugation. Proteins found in the pellet (lane d) bound to MTs, provided that their sedimentation was dependent on the presence of MTs in the assay. Dots in the left margin indicate the positions of molecular mass standards (in kilodaltons from top: 205, 116, 97, 66, 45, 29, respectively), and the dash indicates the position of pp50.
